摘要
以4个不同禽类品种(金定鸭、长乐灰鹅、象洞鸡和闽南火鸡)为试验材料,采用4种不同方法从其全血中提取DNA,比较不同方法的DNA提取效率。结果表明:由于品种之间DNA存在差异,DNA的质量和产率也有显著差异,金定鸭全血DNA用树脂法效果最佳;长乐灰鹅和闽南火鸡全血DNA用酚-氯仿法效果最佳;而盐析法是提取象洞鸡全血DNA的最佳方法。
Four methods were used to extract DNA from blood sample of four poultries （Jinding duck, Changle goose, Xiangdong chicken and Minnan turkey）.Compare the efficiency of various DNA extraction methods on different poultry.The results showed that the best method for DNA extraction from blood sample of different poultry breeds was disparity, and the quality and quantity of DNA was disparity too.The best means of DNA extraction from Jinding duck blood was resin method, the best method for Changle grey goose and Minnan turkey was the classic extraction method and Xiangdong chicken was salting out method.
出处
《福建畜牧兽医》
2008年第2期4-6,共3页
Fujian Journal of Animal Husbandry and Veterinary medicine
基金
福建省科技厅重大专项资助(项目编号:2006NZ0003-3)
