摘要
目的:探讨非小细胞肺癌(nonsmall-cell lung cancer,NSCLC)组织蛋白酶D(cathepsin D,Cath-D)表达与NSCLC发生、发展及浸润转移的关系。方法:肺癌组56例肿瘤组织保存蜡块,其中临床分期Ⅰ期15例,Ⅱ期28例,Ⅲ期13例。病理分级高分化16例,中分化22例,低分化18例。另选择18例癌旁组织作对照。采用免疫组织化学染色法和原位杂交法检测Cath-D表达和mRNA水平。结果:NSCLC组Cath-D定位于癌细胞胞浆,阳性率(41/56,73.2%)显著高于癌旁组(2/18,11.1%),淋巴转移NSCLC中的阳性率显著高于无转移NSCLC的,不同临床分期、不同病理分化程度的NSCLC Cath-D蛋白阳性表达率有显著区别。Cath-D mRNA阳性率(39/56,69.6%)显著高于癌旁组(1/18,5.6%),不同临床分期、不同病理分化程度的NSCLC Cath-D mRNA阳性表达率有显著区别。结论:NSCLC组织中Cath-D和mRNA均有较高水平表达,Cath-D表达与NSCLC的发生、发展及浸润转移有关。
Objective ：To explore the expression of cathepsin D in NSCLC. Methods：56 specimens in patients with NSCLC were included in this experiment.Among them, 15 cases were in clinical stage Ⅰ ,28 in stage Ⅱ and 13 in stage Ⅲ. Another 18 latero-tissue away from NSCLC were included in the control group. The expression of cathepsin D was detected with the technology of immunohistochemistry staining and in situ hybridization. Results ：In NSCLC group,the cathepsin D protein was expressed in the cell cytoplasm,the expressing rate of cathepsin D protein was significantly higher than that in the control group. The expressing rate of cathepsin D protein in the lymphatic metastasis group was significantly higher than that in the no lymphatic metastasis group. The expressing rates of cathepsin D protein were different in different clinical stages of NSCLC. The cathepsin D mRNA was expressed in the cell cytoplasm in the NSCLC group. The expressing rate of cathepsin D mRNA in NSCLC group was significantly higher than that in the control group. Conclusion：Cathepsin D expression plays an important role in the occurrence of NSCLC,which has close relationship with the progress and infiltration of NSCLC.
出处
《现代医药卫生》
2008年第8期1118-1120,共3页
Journal of Modern Medicine & Health
