摘要
目的研究细胞膜脂质流动性和超氧化物歧化酶（ＳＯＤ）在肺结核和肺癌鉴别诊断中的价值。方法采用荧光标记（ＤＰＨ）技术测定膜脂质流动性；采用生物发光法测定ＳＯＤ。结果肺结核、肺癌患者淋巴细胞及肺腺癌和肺鳞癌癌细胞的膜脂质流动性分别为４．０２６±０．７２２、３８．２５４±０．１００、２２．５５７±３．７７１和３２．８７５±９．７０９。肺结核和肺癌患者淋巴细胞的ＳＯＤ含量分别为１７０．７和６５．１４ｍｇ／Ｌ。肺结核和肺癌患者比较，差异统计学上有显著意义（Ｐ＜０．０５）。其中轻、中、重度肺结核患者淋巴细胞的ＳＯＤ含量分别为２７０．８±４．１ｍｇ／Ｌ、１６０．２±１．９ｍｇ／Ｌ和９０．４±１．６ｍｇ／Ｌ，其变化随病情加重而降低。结论膜脂质流动性和ＳＯＤ测定对辅助临床鉴别诊断肺结核和肺癌是有用的手段。
Objective To evaluate the application of membrane lipid fluidity and superoxide dismutase (SOD) in differential diagnosis of pulmonary tuberculosis and lung cancer. Method Fluorescence labeling (DPH) technique was used for detection of membrane fluidity, while biological illumination method for dectection of SOD. Result The membrane lipid fluidity of lymphocytes obtained from broncho alveolar lavage fluid(BALF) in the pulmonary tuberculosis and lung cancer patients, and the membrane lipid fluidity of carcinoma cells in lung adenocarcinoma and squamous cancer patients were found to be 4.026±0.722, 38.254±0.100,22.557±3.771 and 32.875±9.709 respectively, and statistically significant difference was found between pulmonry tuberculosis and lung cancer patients. The contents of SOD were 170.7 mg/L in the pulmonary tuberculosis and 65.4 mg/L in the lung cancer patients, and statistically significant difference was also found between the two groups. The contents of SOD in the mild, moderate and severe tuberculosis patients were 270.8±4.1 mg/L, 160.2±1.9 mg/L and 90.4±1.6 mg/L respectively, and SOD contents decreased with deterioration of clinical status. Conclusion The membrane lipid fluidity and SOD might be useful biological markers for differential diagnosis between pulmonary tuberculosis and lung cancer.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1997年第5期307-310,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
肺结核
肺肿瘤
细胞膜脂质
流动性
SOD
诊断
Tuberculosis
pulmonary
Pulmonary neoplasm
Membrane lipids
Membrane lipid fluidity
Superoxide dismutase
