摘要
【目的】研究宫颈癌恶变过程中血管内皮细胞生长因子C(VEGF-C)及膜突蛋白(moesin)的表达变化,并在培养的宫颈癌细胞中初步探讨VEGF-C对膜突蛋白的调控作用。【方法】利用临床上活体取材的不同临床分期的宫颈癌组织标本及正常对照宫颈组织,蛋白免疫印迹法检测VEGF-C及moesin蛋白的表达情况。体外培养宫颈癌细胞株Hela细胞,蛋白免疫印迹法测定VEGF-C对moesin蛋白的表达和磷酸化的调控作用。【结果】随着宫颈癌临床分期的增高,VEGF-C、moesin蛋白及磷酸化moesin蛋白表达水平均随之增加。与正常宫颈组织比较,宫颈原位癌(CIN)组织中VEGF-C、moesin、磷酸化moesin蛋白表达分别增加了45%±9%、63%±12%、74%±16%(P<0.05);宫颈鳞癌Ⅰ期各蛋白增高幅度分别为94%±18%、104%±27%、123%±30%(P<0.01);宫颈鳞癌Ⅱ期(未放疗化疗)各蛋白表达增高更为显著,其幅度分别为186%±24%、246%±37%、194%±28%(P<0.001)。在培养的宫颈癌细胞株HeLa细胞上,VEGF-C(100#g/L)处理24h后,可显著增高moesin蛋白、磷酸化moesin蛋白的表达。VEGF-C单克隆抗体可抑制VEGF-C对moesin蛋白表达及磷酸化的上调作用。【结论】VEGF-C、moesin蛋白表达与宫颈癌恶性程度密切相关,推测VEGF-C可能通过上调moesin表达及其磷酸化水平而促进宫颈癌的恶性进展。
[ Objective ] To study the status of vascular endothelial growth factor C （VEGF-C） and moesin expression in cervical cancer malignant progression and to investigate the modulatory effect of VEGF-C on moesin expression. [ Methods ] Cervical cancer samples in different pathological stages were taken from patients and cervical cancer HeLa cells were cultured. The expression level of VEGF-C and moesin in sample tissues and cultured cells were detected by Western blot. [Results] The expression levels of VEGF-C and moesin from sample tissues were gradually increased in parallel with the malignant progression of cervical cancer. Compared with control （normal cervix）, the magnitude of increase of VEGF-C, moesin as well as phospho-moesin protein expression in CIN were 45% ± 9%, 63% ± 12%, and 74% ± 16% （P 〈 0.05）, respectively. In cervical squamous carcinoma at stage I, they were increased by a magnitude of 94% ± 18%, 104% ± 27%, and 123% ± 30% （P 〈 0.01）, respectively. These proteins were more highly expressed in cervical squamous carcinoma at stage II, with the enhancement of 186% ± 24%, 246% ± 37%, and 194% ± 28% （P 〈 0.001） respectively compared to control. In cultured cervical cancer HeLa cells, treatment with VEGF-C （100 μg/L） for 24 h enhances the moesin expression and phosphorylation,which is inhibited by VEGF-C monoclonal antibody. [Conclusions] The expression level of VEGF-C and moesin protein are tightly relative to cervical cancer malignant progression. VEGF-C may play the important role in cervical cancer progression through upregulation of moesin expression and phosphorylation.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2008年第2期173-176,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技计划基金(2007B031513008)
关键词
宫颈癌
血管内皮生长因子C
膜突蛋白
cervical cancer
vascular endothelial growth factor C
moesin
