摘要
木醋杆菌在传统的分批静置培养方式下产生的细菌纤维素具有良好的成膜性,但难于实现工业化生产,为了解决这个问题,对传统的静置培养装置进行改造,形成流加静置培养方式,能够将静置培养与动态培养的优点结合起来。对分批静置和流加静置2种培养方式进行比较,结果表明,流加培养时,细菌纤维素产量达到11.7g/L,其产量是分批培养的3.44倍,反应器的效率为0.585 g/(L·d),高于分批培养的0.425 g/(L·d)。流加培养条件下细菌纤维素产量和反应器效率得到提高,主要是由于在整个培养过程中营养供给充足,促进木醋杆菌的生长,使糖转化率及细菌平均比生长速率都达到较高水平,大量转化和合成细菌纤维素。
Bacterial cellulose produced by Acetobacter xylinum in batch culture has good filming property, but the process is hard to scale up into industrial production. In order to solve the problem, practice of producing bacterial cellulose in fed-batch culture was adopted to replace the batch culture. The culture method combines the advantages of static culture and agitated culture. A comparison of two culture methods in production of bacterial cellulose shows that the yield of bacterial cellulose reach 11.7g/L in fed-batch culture, which is 3.44 times more than in the batch culture. The efficiency of fed-batch reactor is 0. 585 g/（L · d）, which is higher than the efficiency of that in batch reactor. This is due to enough nutrition supply in fed- batch culture, which would be helpful for cell growth, sugar conversion, and thus increase the average specific growth and the higher productivity of bacterial cellulose.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第2期21-24,共4页
Food and Fermentation Industries
关键词
木醋杆菌
细菌纤维素
分批静置培养
流加静置培养
Acetobacter xylinum, bacterial cellulose, static batch culture, static fed-batch culture
