摘要
产细菌纤维素能力较强的微生物是木醋杆菌。影响较大的发酵条件有:①培养基中含有乳酸盐或甲硫氨酸,能加速细胞生长,提高纤维素产量。②当氧分压为大气压的10%~15%时,纤维素的产量最高。提高溶氧会使气相的CO2分压同时加大,而降低纤维素的生产速率。③在发酵培养基中加入醋酸作为能源,能保持培养基中pH的稳定,有利于纤维素的生成。④在培养基中添加少量纤维素酶可提高纤维素的产量。分别添加1.5%的乙醇,0.1%的醋酸,0.2%的柠檬酸,纤维素的产量可分别提高59.5%,44.4%和40.5%。(陶然)
Acetobacter xylinum has strong bacteria cellulose-producing capability. And the following fermentation conditions are the key influential factors:①culture medium containing lactate or methionine could accelerate cell growth and further increase cellulose output;②when oxygen partial pressure 10 %~15 % of barometric pressure, cellulose output reached the highest. And increase of dissolved oxygen percentage would also increase gas phase CO2 partial pressure, as a result, production rate of cellulose reduced;③addition of acetic acid in fermented culture medium as energy source could maintain pH stability in culture medium helpful for cellulose generation;④ addition of small quantity of cellulase in culture medium could increase cellulose output. Addition of 1.5 % alcohol or 0.1 % acetic acid or 0.2 % citric acid could increase cellulose output by 59.5 % and 44.4% and 40.5 % respectively. (Tran. by YUE Yang)
出处
《酿酒科技》
北大核心
2005年第1期31-33,共3页
Liquor-Making Science & Technology
基金
天津市自然科学基金项目编号:983606811
关键词
微生物
细菌纤维素
发酵条件
生物合成
木醋杆菌
microbe
bacteria cellulose
fermentation conditions
biosynthesis
Acetobacter xylinum