摘要
目的建立青果颗粒中没食子酸的含量测定方法。方法采用Hypersil BDS C18色母柱（250mm×4．6mm，5μm），甲醇-0．05％磷酸溶液（5：95）为流动相。检测波长为220nm。结果没食子酸在4．0688～203．4400μg之间有良好的线性关系。平均回收率为100．41％（n=6），RSD为1．03％。结论本法操作简便、灵敏、准确、重复性好，可用于青果颗粒的质量控制。
OBJECTIVE To develop a method for determining of gallic acid in Qingguo granules. METHODS Hypersil BDS C18 column （250mm × 4.6mm × 5μm） was used with methanol-0. 05% phosphonic acid （5：95） as mobile phase, detection wavelength at 220nm. RESULTS The liner range was 4. 0688 - 203.44004μg · mL^-1 for gallic acid. The average recovery rate was 100. 41% （n = 6） , with RSD 1.03%. CONCLUSION The method was simple, sensitive and accurate with good reproducibility. This method is available for quality control of Qingguo granules.
出处
《海峡药学》
2008年第1期30-32,共3页
Strait Pharmaceutical Journal
