摘要
以桂花(Osmanthus fragrans Lour.)的休眠芽?萌动芽和嫩枝的茎尖为外殖体,进行离体培养研究,筛选其最适培养基。结果表明:3种外殖体的适宜初代培养基不同,其中:休眠芽为B5+7.0 mg.L-16-BA+0.05 mg.L-1NAA+3%蔗糖;萌动芽为B5+5.0 mg.L-16-BA+0.05 mg.L-1NAA+3%蔗糖;嫩枝为B5+3.0 mg.L-16-BA+0.05 mg.L-1NAA+3%蔗糖;但适宜的继代培养基(B5+2.0 mg.L-16-BA+0.05 mg.L-1NAA+3%蔗糖)和生根培养基(B5+2.0 mg.L-1NAA+3%蔗糖)相同。
The cultural conditions of top buds of dormant buds,germinating buds and young shoots of Osmanthus fragrans Lour.were studied in vitro culture.We finally found out the most suitable media.Firstly,the most suitable initial media are B5＋7.0 mg·L^-1 6-BA＋0.05 mg·L^-1 NAA＋3% sucrose for the dormant buds,B5＋5.0 mg·L^-1 6-BA＋0.05 mg·L^-1 NAA＋3% sucrose for the germinating buds and B5＋3.0 mg·L^-1 6-BA＋0.05 mg·L^-1 NAA＋3% sucrose for the young shoots.Secondly,the most suitable regeneration medium is B5＋2.0 mg·L^-1 6-BA＋0.05 mg·L^-1 NAA＋3% sucrose and the most conducive rooting medium is B5＋2.0 mg·L^-1 NAA＋3% sucrose for all of them.
出处
《西北植物学报》
CAS
CSCD
北大核心
2008年第2期244-248,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30670137)
河南省高校创新人才培养基金
关键词
桂花
茎尖
离体培养
Osmanthus fragrans
top buds
in vitro culture
