摘要
目的：建立柱后衍生-高效液相色谱法测定保健食品、强化食品和营养素补充剂中的叶酸。方法：样品经水提取,选用乙腈＋50 mmol/L磷酸二氢钾溶液（pH=3.5）=12＋88为流动相,流速为1.0 ml/min,经Kromasil C18柱分离（柱温为常温）,用0.5%的过二硫酸钾为衍生剂,流速0.3 ml/min,反应温度60℃,使用荧光检测器,Ex=365 nm,Em=450 nm。结果：被测组分浓度（0.010～2.0μg/ml）与峰面积线形关系良好（r=0.9999）;加标回收率为92.11%～95.31%,相对标准偏差（RSD）2.99%～3.44%。结论：该方法操作简便、快速、测定结果准确可靠,适合保健食品、强化食品和营养补充剂中的叶酸测定。
Objective：A quantitative method was developed for the analysis of folic acid in health foods, supplementary foods by high performance liquid chromatographic method with post - column chemical derivation and fluorescence detection. Methods： After being extracted with water in an uhrasonica,a Cls column was used for separation at room temperature,and elution was performed with acetonitrile ＋ potassium dihydrogen phosphate（ pH = 3.5） = 12 ＋ 88 at 1.0 ml/min. Folic acid in the eluate was deri- vatized to an intensive fluorescence compound by 0, 5% potassium peroxydisulfate at 60℃ and detected by a spectrofluorimetor （Ex = 365 nm, Em = 450 nm） at 0. 3 ml/min. Results： A linear calibration curve was obtained in the range of 0. 010 -2. 0μg/ml （ r = 0. 9999） ,RSD were 2. 99% - 3.44% and the recoveries of spiked analyte were in the range of 92. 11% - 95. 31%. Conclusion：The developed method can be applied to determine the folic acid contents in health food and supplementary foods. The method is rapid, simple, accurate and reliable.
出处
《中国卫生检验杂志》
CAS
2008年第2期268-269,294,共3页
Chinese Journal of Health Laboratory Technology
关键词
高效液相色谱
叶酸
High performance liquid chromatography
Flolic acid
