摘要
目的:探讨慢性乙型肝炎前C区终止变异的临床意义。方法:建立一种简单而灵敏度高的选择性聚合酶链反应(PCR)检测乙型肝炎病毒前C终止变异。结果:在47例慢性乙型肝炎中检出前C终止变异38例(81％),其在慢性肝炎、肝硬化、慢性重型肝炎中的检出率分别为82％(28/34)、80％(8/10)和67％(2/3),在HBeAg日性和抗HDe阳性中分别为76％(13/17)和83％(25/30),P>0.05。结论:前C终止变异在HBeAg阳性慢性肝病中亦呈高频率检出,抗HBe阳性慢性肝病并不都与前C终止变异有关。
Aim: To study the. clinical significance of chronic hepatitis B precore stop codon mutant. Method: A convenient and sensitive method of selective polymerase chain reaction has been developed. Result: The presence of precore stop mutants was totally detected in 38 of 47(81%) chronic hepatitis B (CHB) by the method. The prevalence in chronic hepatitis, cirrhosis and chronic advanced hepatitis was 82% (28/34 ), 80% (8/10) and 67% (2/3) respectively. The. presence of precore stop mutants was detected in 13 of 17 (76% ) HBeAg positive and 25 of 30 (83%) anti-HBe positive CHB (P>0. 05). Conclusion: The results show that there was an high frequence of precore stop mutant in CHB with HBeAg-positive, and the anti-HBe positve chronic hepatitis B did not a always present the precore stop mutant.
出处
《中西医结合肝病杂志》
CAS
1997年第3期135-137,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
