摘要
应用突变特异性ＰＣＲ（ｍｕｔａｔｉｏｎｓｐｅｃｉｆｉｃＰＣＲ简称ｍｓＰＣＲ）技术对１１７例１４２份临床各型乙肝病毒（ＨＢＶ）感染者的血清标本进行了ＨＢＶ基因前ｃ区１８９６位已知Ｇ→Ａ点突变株的检测，发现２４例３０份标本有突变株感染，检出率为２０．５１％。其中重肝组３９．１３％（９／２３），慢活肝组１９．６４％（１１／５６），慢迁肝组１３．３３％（２／１５），无症状携带者组８．６９％（２／２３）。分析结果发现ＨＢＶ基因前Ｃ区突变株感染与重肝的发生、发展有一定的关系，对干扰素的疗效似有影响，与年龄，性别差异关系不大。
42 serum samples from ll7 cases with chronic HBV infections
have been screemed for G-A mutant at 1896 site on HBV pre-C region
using the method of 3'-end mutation specific PCR。 30 samples from 24
cases were infected with this kind of mutant, positive rate was
20.52%。Among these cases, 39.17% was of fulmi-nant hepatitis(9/23),
19.64%(11/56)for CAH and l3.33%(2/23) for CPH, The results showed
that there was some ralationships between HBV pre-C mutation and
fulminant hepatitis, and the effect of in terferon treatment would be
influenced by thls kinds of HBV muation。
出处
《肝脏病杂志》
CSCD
1994年第3期138-140,共3页
