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我国部分兰属植物菌根真菌rDNA ITS序列分析 被引量:24

rDNA ITS Analysis of Mycorrhizal Fungi in Cymbidium Plants
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摘要 rDNA internal transcribed spacer analysis(rDNA ITS)was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains,which were isolated from Chinese orchids(Cymbidium)distributed in different sites and belonged to different ecologic types.The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella,which was fully consistent with identification as Epulorhiza with morphological method.Additionally,based on rDNA ITS sequence cluster analysis,dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificity-relation between Chinese orchids(Cymbidium)symbiotic mycorrhizae and hosts. rDNA internal transcribed spacer analysis (rDNA ITS) was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains, which were isolated from Chinese orchids ( Cymbidium ) distributed in different sites and belonged to different ecologic types. The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella, which was fully consistent with identification as Epulorhiza with morphological method. Additionally, based on rDNA ITS sequence cluster analysis, dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificity-relation between Chinese orchids (Cymbidium) symbiotic mycorrhizae and hosts.
出处 《林业科学》 EI CAS CSCD 北大核心 2008年第2期160-164,共5页 Scientia Silvae Sinicae
基金 ‘948’项目(2004-4-26) ‘948’项目(2005-4-37) 北京市属市管高校人才强教计划资助项目
关键词 兰属植物 菌根真菌 RDNA ITS分析 瘤菌根菌 胶膜菌属 Cymbidium mycorrhizal-fungi rDNA internal transcribed spacer analysis Epulorhiza Tulasnella
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