摘要
采用三波长-分光光度法测定千山刺五加叶和果中黄酮的含量,消除了由于刺五加叶和果中黄酮光谱吸收峰不对称给定量分析造成的影响,并校正了由于干扰组分的吸收光谱具有线性吸收产生的基线倾斜。结果表明:回归方程ΔA=88.795C-0.2060;相关系数r=0.9994;黄酮浓度在0～40.6μg/ml范围内,分别在波长λ1=463nm、λ2=417nm、λ3=382nm测吸光度时,则ΔA与浓度C之间呈良好的线性关系,可按标准曲线法进行定量分析。本法的回收率为96.86%～102.46%;变异系数小于0.136%。方法的准确度与精密度均令人满意,而且操作简便易行。
Determination of flavonoids in Qianshan Acanthopanax senticosus leaves and fruits by three-wavelength spectrophotometry can eliminate the absorbance error of interfering components, turbid solution and the scattering effect. Background changed with concentration changed and asymmetric absorption peak problems can be solved also. The regression equation of concentration A A was obtained： A A =88.795C-0.2060 with a relation coefficient）, r=0.9994. The experimental results demostmte the total flavonoids concentrations in 0～40.6 6μg/ml with A A obeying linear relation. When the absorbanee was measured at wavelength λ1=463 nm, λ2=417 nm and λ3=382 nm. The recovery is 96.86%～102.46% and the coeficient of variation is 0.136%. The method is more advantageous than tranditional spectrophotometry method.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第3期393-395,共3页
Food Science
基金
辽宁省教育厅科学研究计划资助项目(200502)
关键词
黄酮类化合物
千山刺五加
叶
果
三波长-分光光度法
flavonoids
Qianshan Acanthopanax senticosus
leaves
fruits
three-wavelength spectrophotometry
