摘要
目的本研究通过制作不同幼龄大鼠骨骼染色透明标本来探讨大鼠骨骼染色透明标本制作的基本方法，为实验动物透明标本的制作和普及奠定基础。方法随机抽取生后8～23d的大鼠为研究对象，按照脱水、净化、再脱水、氢氧化钾处理、染色、透明、保存等步骤制作大鼠骨骼染色透明标本。结果制作好的大鼠透明标本肌肉和软组织结构无色透明，骨骼界限明显，易于识别，其中第8天大鼠的透明效果明显优于第23天大鼠。结论新生大鼠骨骼染色透明标本的制作与其它动物的透明标本制作有明显不同，且透明效果与鼠龄相关。
Objective Different neonatal rats＇transparent stain rat skeletal specimens were made to explore the basic method of making laboratory animal＇s transparent sample. Methods Neonatal rats aged from 8 clays to 23 clays were selected randomly, according to dehydration, purification, dehydration, potassium hydroxide, dyeing, transparent, and other steps, the rat skeletal specimens were made. Results The making good specimens had easily identifiable muscle and soft tissue, the transparent effect of the eight clays was better than the 23 days. Conclusion The procedure of neonatal rat specimen had difference with other animals, and the effects of transparent associated with the age.
出处
《滨州医学院学报》
2008年第1期14-15,共2页
Journal of Binzhou Medical University
基金
滨州医学院大学生科技创新活动基金项目资助(BY2006DKCX13)
关键词
新生大鼠
骨骼染色
透明标本
neonatal rat
bone stain
transparent sample
