摘要
以中性红为光谱探针,采用荧光光谱和紫外-可见光谱法研究了葛根素与DNA的相互作用,考察了离子强度和I-离子浓度对葛根素与DNA作用的影响。结果表明,在生理条件下(pH 7.4),葛根素通过沟槽结合方式与DNA发生作用,DNA对葛根素荧光猝灭属于静态猝灭,测得其结合常数为4.81×104L/mol。
The interaction between puerarin and DNA was studied by fluorescence and absorption spectrometric methods using neutral red as a spectral probe. Effect of ionic strength and I^- concentration on the binding of puerarin with DNA were investigated. It is found that puerarin can react with DNA under physiological condition （pH 7.4）. The major mode of recognition is groove binding between puerarin and DNA. The quenching belonged to static fluorescence quenching. The binding constant for puerarin with DNA was 4.81×104 L/mol.
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第3期1-4,共4页
Chinese Journal of Analysis Laboratory
基金
教育部长江学者和创新团队发展计划(IRT0540)
江西省教育厅基金(赣教技字[2005]-71号)项目资助
