摘要
应用荧光光谱法研究三种原小檗碱类生物碱:盐酸小檗碱(Berberine hydrochloride)、药根碱(Jatrorrhizine)和盐酸巴马汀(Palmatine hydrochloride)与抗凋亡蛋白Bcl-2之间的相互作用,探讨了这三种生物碱对Bcl-2蛋白的荧光淬灭机理,分别测定其结合常数和结合位点数.实验结果表明,盐酸小檗碱、药根碱和盐酸巴马汀等三种原小檗碱类生物碱都能够使Bcl-2蛋白的内源性荧光发生淬灭,其荧光淬灭过程是静态淬灭过程.通过比较三种生物碱与Bcl-2蛋白的相互作用,发现随着分子柔性增强,其与Bcl-2蛋白的结合能力增强.因此,增强生物碱的分子柔性有可能增强其靶向Bcl-2蛋白的能力.
The interactions between Bcl-2 and three protoberberine alkaloids： berberine hydrochloride,jatrorrhizine and palmatine hydrochloride were studied by fluorescence spectroscopy.The binding constants and the number of binding sites were measured,respectively,and the quenching mechanism was proposed.The results indicated that the protoberberine alkaloids could decrease the intrinsic fluorescence of Bcl-2 mainly through static quenching.By comparing the structures of the three alkaloids,it was found that the binding affinity to Bcl-2 protein increased with molecular flexibility of the alkaloids.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2011年第2期247-251,共5页
Acta Chimica Sinica
关键词
盐酸小檗碱
药根碱
盐酸巴马汀
BCL-2
荧光光谱
berberine hydrochloride
jatrorrhizine
palmatine hydrochloride
Bcl-2
fluorescence spectros-copy
