摘要
目的:探讨多房棘球绦虫重组BCG-Em14-3-3疫苗免疫和Em原头节攻击后小鼠脾细胞因子的变化。方法:将疫苗采用皮下注射和鼻腔内接种分别免疫BALB/c鼠,免疫后8周用多房棘球绦虫原头节进行攻击感染,感染后18周杀鼠取脾,分离脾细胞,用EmAg或ConA刺激培养,收集脾细胞培养上清液,用试剂盒检测脾细胞培养上清液的IL-2I、FN-γ、TNF-α和IL-4水平,同时设有空载体、BCG和PBS对照。结果:疫苗接种组的IFN-γ和TNF-α水平升高,IL-4水平降低;鼻腔内接种组的TNF-α水平高于皮下注射组。结论:多房棘球绦虫重组BCG-Em14-3-3疫苗诱导小鼠产生一个TH1型反应,从而对抗Em原头节攻击感染。
Objective：To investigate the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine of Echinococus muhilocularis （Em） and challenge with Em protoscoleces. Methods： BALB/c mice were subcutaneously and intranasally vaccinated respectively,then challenged with Em protoscoleces at 8th week of vaccination and killed at 18th week of infection to get spleen.Splenocytes were separated to culture by stimulation with EmAg or ConA.The supernatants were collected to measure IL-2,IFN-γ,TNF-αand IL-4 by ELISA Kits,and blank vector,BCG and PBS served as control. Results：In the groups of immunization,levels of IFN-γ and TNF-α increased, but that of IL-4 decreased.The level of TNF-α in the intranasal group was higher than that in the subcutaneous group. Conclusion：Tsl response is induced in mice by rBCG-Em14-3-3 vaccine which is against the challenge of Em protoscoleces.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第1期9-12,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金(30671835
30500423和30200239)
教育部重点项目(205131)
重庆市教委科研基金(KJ050313)
重庆市科委科研基金(03-43-8)
重庆市卫生局科研基金(03-2-099)
