摘要
目的:检测急性冠脉综合征(acute coronary syndromes,ACS)患者外周血单核细胞血凝素样氧化低密度脂蛋白受体-1(lectin-like oxidized low-density lipoprotein receptor-1,LOX-1)的表达水平,以及抗炎因子白介素-10(interleukin-10,IL-10)对其表达的影响。方法:收集28例健康体检者和28例ACS患者,用酶联免疫吸附法(enzyme-linked immunosorbnent assay,ELISA)检测血清中IL-10和肿瘤坏死因子(tumor necrosis factor,TNF-α水平。分离外周血单核细胞进行培养,加或不加IL-10(20μg/L)进行体外干预,检测单核细胞LOX-1蛋白和mRNA表达的变化。结果:与健康对照组相比,ACS患者血清中IL-10和TNF-α水平明显升高(P<0.01)。ACS患者外周血单核细胞LOX-1蛋白和mRNA表达明显高于对照组(P<0.01),IL-10(20μg/L,3h)可明显下调单核细胞LOX-1的蛋白和mRNA表达(P<0.01)。结论:抗炎因子IL-10可能通过抑制ACS患者外周血单核细胞LOX-1的表达而起到稳定斑块的作用,这可能是IL-10抗动脉粥样硬化的又一新机制。
Objective To determine the expression of lectin-like oxidized low-density lipopro- tein receptor-1 （LOX-1） of peripheral blood monocytes in patients with acute coronary syndromes （ACS） , and to further explore the effect of anti-inflammatory factor interleukin-10 （IL-10 ） on the expression of LOX-1. Methods Twenty-eight healthy controls and 28 ACS patients were enrolled in the study. The levels of IL-10 and tumor necrosis factor （TNF-α） were determined by enzymelinked immunosorbnent assay （ELISA）. The monocytes of peripheral blood in patients and controls were isolated and incubated with exogenous IL-10 （20 μg/L）. The expression of LOX-1 protein and mRNA in the monocytes was examined by Western blot and reverse transcriptase PCR （RT- PCR）. Results Compared with the healthy controls, the levels of serum IL-10 and TNF-α were significantly elevated in ACS patients （ P 〈 0.01 ）. The expression of LOX- 1 protein and mRNA was markedly upregulated in the isolated monocytes from ACS patients, which could be downregulated by IL- 10 （ 20 μg/L, 3 h ） （ P 〈 0.01 ）. Conclusion The effect of anti- inflammatory factor IL- 10 on the atherosclerosis may be a new mechanism resulting in plaque stabilization via the decreased LOX-1 expression of peripheral monocytes in ACS patients.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2008年第2期169-173,共5页
Journal of Central South University ：Medical Science
