摘要
目的:检测CD44V6、β-catenin在骨肉瘤组织中的表达,分析其表达与人骨肉瘤细胞侵袭及转移能力的相关性。方法:应用免疫组织化学方法检测35例骨肉瘤、15例骨软骨瘤、15例正常骨组织中CD 44V6、β-catenin的表达。结果:骨肉瘤组织中CD 44V6、β-catenin的阳性表达显著高于骨软骨瘤及正常骨组织(P<0.01);两者的表达分别与骨肉瘤临床分期、肿瘤转移及2年内死亡有关(P<0.05),而与患者性别、年龄、肿瘤大小、病理分型、术前化疗无关(P>0.05);两者的阳性表达呈正相关(P<0.01)。结论:CD 44V6、β-catenin在骨肉瘤中的阳性表达与骨肉瘤的发生、发展和转移密切相关,可能在骨肉瘤细胞侵袭、转移的过程中起协同作用;联合检测CD 44V6、β-catenin可以作为骨肉瘤诊断及其转移预测的指标。
Objective：To detect the expressions of CD 44V6 and β-catenin in osteosarcoma and to analyze the association of their expressions with migration and invasion of osteosarcoma cells. Methods：Expressions of CD 44V6 and β-catenin were examined by immunohistochemical SP method in 35 specimens of human osteosarcoma tissues, 15 specimens with osteochondroma, and 15 cases of normal bone tissues. Results：The positive expressions of CD 44V6 and β-catenin were significantly higher in osteosarcoma than osteochondroma and normal control tissues （P〈0.01）. Their expressions significantly correlated with clinical staging, tumor metastasis, and 2-year death （P〈0.05）; but had no correlation with the age and gender of patients, tumor size, histological classification, and with or without preoperative chemotherapy （P〉0.05）. In osteosarcoma, expression of CD 44V6 was positively associated with the expression of β-catenin （P〈0.01）. Conclusion：The positive expressions of CD 44V6 and β-catenin closely correlate with the occurrence, development, and metastasis of osteosarcoma. They may play synergistic role in the invasion and metastasis of osteosarcoma. Combined detection of CD 44V6 and β-catenin may be used as an indicater for diagnosis and prognosis of metastasis of osteosarcoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2008年第2期136-138,共3页
Tumor
关键词
骨肉瘤
基因表达
抗原
CD44
Β-连环素
肿瘤侵袭
肿瘤转移
Osteosarcoma
Gene expression
Antigens,CD 44
Beta catenin
Neoplasm invasiveness
Neoplasm metastasis
