摘要
目的为了探索单疱病毒性角膜炎（ＨｅｒｐｅｓＳｉｍｐｌｅｘＫｅｒａｔｉｔｉｓ，ＨＳＫ）的发病机制和快速诊断ＨＳＫ。方法应用多聚酶链反应（ＰｏｌｙｍａｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ，ＰＣＲ）对感染ＨＳＫ的２０只纯新西兰白兔和６０例ＨＳＫ患者的角膜进行了单疱病毒－Ⅰ（ｈｅｒｐｅｓｓｉｍｐｌｅｘｖｉｒｕｓ－Ｉ，ＨＳＶ－Ｉ）ＤＮＡ检测。结果急性感染期的５只兔角膜和１２只人角膜均阳性；用稳定期４５ｄ后的兔角膜供体行部分穿透性角膜移植术，术后５０ｄ１０只兔角膜中７只阳性；稳定期６个月～６年的１８只人角膜片，１２只阳性；３０例临床可疑ＨＳＫ角膜刮取物，２６例阳性；５只未感染ＨＳＫ的兔角膜均阴性。结论角膜可能是ＨＳＶ－Ⅰ的另一潜伏基地；ＰＣＲ可作为一种临床上检测ＨＳＶ的方法，具有快速、准确的特点，值得推广。
Objective To investigate the recurrence mechanism and fast diagnosis of herpes simplex virus keratitis(HSK).Method Using PCR,to give a HSV Ⅰ DNA inspection to the cornea of 20 pure New Zealand rabbits which have infected with HSK and 60 patients who also infected wich HSK.Result 5 corneas of rabbits and 12 corneas of person which are in acute infection period are positive;Use rabbit cornea donor gave PKP after 45 days of steady period,seven corneas are positive 50 days after operation on 10 corneas;12 corneas are positive among 18 person corneas of 6 months～6 years of steady period;26 cases are positive among 30 clinical suspicions HSK corneas craped substances;all the 5 rabbits corneas which have not infected with HSK are negative.Conclusion Prompt us that corneas may be another latent base of HSV Ⅰ;PCR can be used as a method of inspecting HSK,it has the chatacter of accurate and quick which is worth of popularizing.
出处
《潍坊医学院学报》
1997年第3期174-175,共2页
Acta Academiae Medicinae Weifang
关键词
单纯疱疹病毒
角膜
基因诊断
多聚酶链反应
herpes simplex virus
cornea
human rabbit
gene Polymerase chainreaction
gene
