摘要
目的探索激活态雪旺细胞信号转导通路的分子构成。方法用改良成年SD大鼠雪旺细胞培养法获得激活态和正常态雪旺细胞，分别用RTK和G蛋白耦联的信号转导通路中的7个抑制剂后，Western Blot法检测细胞内的p-ERK1／2水平的改变。比较激活态和正常态雪旺细胞的信号转导通路的差异。结果Western Blot法检测结果显示，在激活态雪旺细胞，使用抑制剂CTX、D609和BAPTA-AM后细胞内的p-ERK1／2水平显著降低（P〈0．01），而其它抑制剂使用后细胞内的p-ERK1／2水平无明显的改变。在正常态的雪旺细胞，使用抑制剂U73122和BAPTA-AM后细胞内的p-ERK1／2水平显著降低，而其它抑制剂使用后细胞内的p-ERK1／2水平无明显的改变，差异有统计学意义（P〈0．01）。结论激活态雪旺细胞和正常态雪旺细胞都是通过G蛋白耦联信号转导通路来发挥生物效应，但是激活态雪旺细胞通过PC-PLC分解磷脂酰胆碱使细胞内的DAG水平的提高来发挥生物效应．而正常态雪旺细胞则通过PI—PIE信号转导通路发挥其生物效应。
Objective To explore molecular composition of activated Sehwann cells＇ signal transduetion pathway. Methods Activated Sehwann cells and normal Sehwann cells were obtained with modified Sehwann cell culture method from adult SD rats. Seven signal tranaduction pathway inhibitors of RTK and GPCR were added into activated Sehwann cells and normal Sehwann cells for an hour respectively. Western Blot was used to detect the change of p-ERK1/2 level and compare the difference between activated Sehwann cells and normal Sehwann cells. Results In activated Sehwann cells, the changes of p-ERK1/2 inhibitor CTX , D609 and BAPTA-AM were prominent whereas there was no distinct change using other inhibitors （ P 〈 0.01 ）. However in normal Sehwann ceils, the changes of p-ERK1/2 inhibitor 1573122 and BAFTA/EDTA were notable whereas there was no distinct change using other inhibitors （ P 〈 0.01 ）. Conclusion GPCR was the common signal transduction pathway of activated Sehwann cells and normal Sehwann cells. Activated Sehwann cells exert their biologic effect through PC-PLC decompounding choline and boosting level of DAG. However normal Sehwann cells exert their biologic effect through PI-PLC signal tranaduction pathway.
出处
《中华手外科杂志》
CSCD
北大核心
2008年第1期45-47,共3页
Chinese Journal of Hand Surgery
基金
国家973计划课题基金资助项目（2005CB522604）
国家自然科学基金资助项目（30470659）
高等学校博士学科点专项科研基金资助项目
