摘要
目的:研究麝香水溶物对培养的大鼠神经干细胞的生长、分化和电转染率的影响。方法:在培养基中加入不同浓度的麝香水溶性提取物后,观察大鼠神经干细胞的生长分化情况;利用表达增强型绿色荧光蛋白的质粒pEGFP-C1,对麝香水溶物处理的神经干细胞进行电穿孔转染,调查电转染率。结果:麝香水溶物处理后的大鼠神经干细胞的细胞团分散,神经突起增多、变长,贴壁细胞增加,细胞形态呈多样性。在0.3‰浓度下,神经干细胞有向神经胶质样细胞分化的趋势。对于麝香处理后变化的细胞,再转到正常培养基中后,细胞基本都能恢复到正常的神经干细胞形态,浓度较高(3‰)时,细胞的恢复能力下降,部分细胞因细胞膜受损严重而死亡。电转染结果表明,麝香处理后发生变化的细胞对pEGFP-C1的电转染率明显提高。结论:麝香水溶物能促进大鼠神经干细胞团的分散和细胞贴壁、变形,并有向神经胶质样细胞分化的趋势。同时可以提高神经干细胞对pEGFP-C1的电转染率。
Aim： To investigate the effect of musk soluble components on the growth, the differentiation and the transfection efficiency of rat neural stem cell （NSC） in vitro. Methods： The growth and the differentiation of rat NSC were observed when musk soluble compo- nents were added into the culture medium of NSC. Meanwhile, the pEGFP-C1, which expressed the enhanced GFP protein, was transfected into the NSC by method of electro- transfection. Results： When NSC was treated with musk soluble components, the neurites were outgrowth around NSC and attached to the plate, and the neural spheres were disassociated. The gila-like cells appeared at the concentration of 0.3%0. When the concentration of musk soluble components was lower than 3%o, the transformative cells could recover. Furthermore, the efficiency of transfection pEGFP into NSC was remarkably increased after the treatment with musk.Conculsion： After the treatment of NSC with musk soluble components, the neural spheres were disassociated, and then attached to the plate. Musk soluble components could induce NSC differentiation into gila-like cells and improve the transfection efficiency of pEGFP-C1 in vitro.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2008年第1期25-28,共4页
Chinese Journal of Applied Physiology
基金
上海市教委科研基金一般项目(06AZ056)
关键词
神经干细胞
麝香
生长
分化
电转染
neural stem cells （NSC）
musk
growth
differentiation
transfection
