摘要
以蝴蝶兰叶片为试验材料,研究热激处理在抑制组织褐变中的作用。采用45℃条件下热激处理10 min,再经过常温下的不同恢复时间后,对叶片切割接种,然后在不同培养时间,对组织的褐化指数、总酚含量以及PAL等褐变相关指标进行测定比较。结果表明,采用热激处理的蝴蝶兰,再经过常规培养条件(25℃)培养48 h后,进行切割接种,可显著减轻随后培养过程中的褐变程度。
To clarify the effect of heat shock on tissue browning of Phalaenopsis, the post-subculture changes in browning index, total phenolic content and PAL activity in tissues recovered under 25 ℃ for different time after heat shock treatment at 45 ℃ for 10 rain were determined and analysed. The results suggested that the browning of cultured Phalaenopsis tissue could be significantly inhibited by heat treatment when the treated tissue was covered for 48 h under 25 ℃.
出处
《园艺学报》
CAS
CSCD
北大核心
2008年第1期143-146,共4页
Acta Horticulturae Sinica
基金
国家‘863’项目(2004AA241200)
国家‘十五’科技攻关计划项目(2004BA521B02)
关键词
蝴蝶兰
组织培养
褐变
热激处理
恢复时间
酚类
苯丙氨酸解氨酶
Phalaenopsis
tissue culture
browning
heat shock
restoration time
phenols
phenylala- nine ammonia-lyase
