摘要
目的检测Skp2、p27kip1和E-cad在卵巢上皮性肿瘤中的表达情况及其相互关系。方法采用免疫组化SP法,检测99例卵巢上皮性肿瘤组织中Skp2、p27kip1及E-cad的表达。结果p27kip1在卵巢良性肿瘤、交界性肿瘤的表达率分别为76.5%、70.6%高于卵巢癌29.2%(P<0.05),在早期癌中的表达高于晚期癌(P<0.05)。Skp2在卵巢良性肿瘤、交界性肿瘤的表达率分别为0、23.5%,均低于卵巢癌50.8%(P<0.05),在早期癌的表达低于晚期癌(P<0.05)。卵巢癌中Skp2表达与组织分化有关,在低分化癌的阳性表达率高于高分化癌(P<0.05),在组织学类型方面,浆液性癌中skp2的阳性表达率高于非浆液性癌(P<0.05)。E-cad在良性肿瘤、交界性肿瘤和卵巢癌表达率分别为88.2%、82.4%、55.4%,恶性组低于良性组(P<0.05)。E-cad在早期癌的表达率高于晚期癌(P<0.05)。Skp2表达与p27kip1表达呈负相关(P<0.05),E-cad表达与p27kip1表达呈正相关(P<0.05),而E-cad表达与Skp2表达则呈负相关(P<0.05)。结论Skp2过度表达与p27kip1表达减少可能与卵巢上皮性肿瘤的发生发展密切相关,而E-cad在晚期卵巢癌中表达降低可能反映了卵巢癌分化程度的降低。
Purpose To examine the expression of Skp2, p27~ipl and E-cadherin in epithelial ovarian tumors. Methods SP immuno- histochemical technique was used to evaluate the expression of Skp2, p27kipl and E-cadherin in 99 cases of epithelial ovarian tumors, respectively. Results For p27kipl , the positive rates of p27kipl were 66. 7% and 70.6% in benign ovarian tumors and borderline tumors, respectively, higher than adenocarcinomas 29.2% （P 〈 0. 05）. A higher detection rate of p27kipl expression was observed in early-stage diseases, compared with advanced-stage diseases （ P 〈 0. 05 ）. The positive rates of Skp2 were 0 and 23.5% in benign ovarian tumors and borderline tumors, respectively, less than adenocarcinomas 50.8% （P 〈 0. 05）, and the frequency of Skp2 expression in advanced-stage diseases was higher than in early-stage diseases （ P 〈 0. 05）. In adenocarcinomas, a higher detection rate of Skp2 expression was observed in low histological grades than in high ones. The rates of Skp2 detection were higher in serous groups than innon-serous groups （ P 〈 0. 05）. The positive rates of E-cadherin were 88.9%, 82.4% and 55.4%, respectively and more less in adenocarcinomas than in benign tumors. A significantly higher detection rate of E-cadherin expression was observed in early-stage diseases compared with advanced-stage diseases （P 〈0. 05）. There was a significantly negative correlation between Skp2 and p27kipl expression, a significantly positive correlation between E-cadherin and p27kiplexpression and a significantly negative correlation between E-cadhefin and Skp2 expression in the group of adenocarcinomas. Conclusions Skp2 overexpression and decreased expression of p27kipl might play an important role in the development and progression of epithelial ovarian tumor. The down-regulation of E-cadherin expression in the advanced stage might reflect poor differentiation.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2007年第6期673-677,共5页
Chinese Journal of Clinical and Experimental Pathology
