摘要
目的探讨异丙酚对24h异相睡眠剥夺大鼠海马谷氨酸（Glu）和γ-氨基丁酸（γ-GABA）释放的影响。方法健康雄性SD大鼠16只，成功完成24h异相睡眠剥夺实验后，随机分为2组（n=8），自然睡眠组（N组）腹腔注射5％脂肪乳剂3ml，异丙酚组（P组）腹腔注射异丙酚100mg／kg。于异相睡眠剥夺前（基础状态）、异相睡眠剥夺24h时（T1）、异相睡眠剥夺结束后1h（T1）、3h（T3）和6h（T4）收集2组海马微透析液，检测Glu和γ-GABA的浓度。结果与基础值比较，N组和P组T1-3，时海马微透析液内Glu、γ-GABA浓度升高，N组T4时上述指标仍较高；与T1时比较，N组和P组T3-4时上述指标降低（P〈0．05或0．01）。两组间Glu、γ-GABA浓度差异无统计学意义（P〉0．05）。结论异丙酚麻醉对大鼠异相睡眠剥夺时海马内紊乱的Glu和γ-GABA递质有恢复作用，其作用与自然睡眠相似。
Objective To investigate the effect of propofol on the release of glutamate and γ-amino-butyric acid （GABA） in hippocampus in rats during recovery from 24 paradoxical sleep deprivation （PSD）. Methods Sixteen healthy male SD rats weighing 250-300 g were used in this study. The animals were anesthetized with intraperitoneal （IP） chloral hydrate 300 mg/kg. A catheter with guide wire was inserted into the CA1 region of hippocampus for micro-analysis and fixed to the skull. The animals underwent 24 h PSD after 4-day adaptation training and were then randomly divided into 2 groups （ n = 8 each） ： Ⅰ natural sleep group （N） received 5% fat emulsion 3 ml IP and was allowed to sleep naturally; Ⅱ propofol group （P） received propofol 100 mg/kg IP. The micro-analysis samples were collected before （T0 , baseline） , at the end of （T1 ） and at 1, 3, 6 h after 24 h PSD （T2-4 ） for determination of glutamate and GABA concentrations using high performance liquid chromatography （HPLC）. The animals were killed at the end of the experiment. The micro-catheter was removed and the hippocampi were isolated and sliced to verify the position of the catheter tip under microscope. The animal was excluded if the catheter was misplaced. Results The concentrations of glutamate and GABA in the micro-analysis samples were significantly increased at the end of 24 h PSD as compared with the baseline values in both groups and were then gradually decreasing and reached the lowest level at 6 h after 24 h PSD （ T4 ） but were still higher than the baseline level in group N. Whereas in group P the glutamate and GABA concentrations at T4 were not significantly different from the baseline values. Conclusion Propofol contributes to the recovery from PSD-induced alterations in Glu and GABA release in hippocampus like natural sleep.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2008年第1期7-9,共3页
Chinese Journal of Anesthesiology
关键词
二异丙酚
海马
睡眠剥夺
谷氨酸
受体
GABA
Propofol
Hippocampus
Sleep deprivation
Glutamic acid
Receptors, GABA
