摘要
运用免疫组织化学技术和分子杂交技术研究了膀胜移行细胞癌表皮生长因子受体（EGFr）表达状况以及EGFr基因扩增和其mRNA与受体蛋白表达的相关性。结果表明：4例正常膀胱粘膜EGFr染色阴性；56例膀胱肿瘤中，29例呈不同程度的阳性染色，并与肿瘤的分期、分级有显著性差异；13例膀胱肿瘤中，EGFr基因未见扩增，5例mRNA过表达，7例EGFr过表达，但二者表达的量并无相关性。提示EGFr表达可作为膀胱肿瘤恶性标志；EGFr过表达可能系基因转录或调控机理失常所引起。
The expression status of epidermal growth factor receptor (EGFr) was evaluated and the relation between amplification and expression of EGFr gene was studied by immunohistochemistry method and molecular hybridization in the urinary bladder transitional cell carcinomal (TCC)- The results showed EGFr staining was negative in the normal urothelium, while positive to varying degrees in 29 of 56 cases of the malignance; there existed significant differences between the expression of EGFr and stage and grade, there was no amplification of EGFr gene, 5 overexpression of EGFr mRNA, 7 overexpression of EGFr of 13 cases of the urinary bladder TCC. It suggested EGFr exPression may be used as a malignance marker of the b1adder tumor and the overe-xpression of EGFr maybe ensued from transcription and transtlation disorders of EGFr gene.
出处
《临床泌尿外科杂志》
1997年第3期179-182,共4页
Journal of Clinical Urology
关键词
膀胱肿瘤
移行细胞癌
EGFγ
免疫组织化学
Transitional call carcinoma of bladder
EGFr
Immunohistochemistry
Molecular hybridization
