摘要
目的:探讨胆固醇7α-羟化酶(cholesterol 7α-hydroxylase,CYP7A1)基因多态性与湖北儿童血脂水平的关系。方法:采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)对216例健康儿童CYP7A1基因的A-204C基因座进行Eco31I酶切多态性分析,血脂测定在全自动化分析仪上进行。结果:研究人群中CYP7A1基因的3型频率分别为:AA型6.0%,AC型26.4%、CC型67.6%。AA基因型在LDL-C,TC,Lp(a)水平上均低于其他基因型,并且其差异具有显著性。CC基因型在HDL-C水平上均高于其他基因型,并且其差异具有显著性。3型在ApoA上两两之间均存在显著性差异,而在TG中无显著性差异。结论:CC基因型在研究人群中所占的比例最高,AA基因型所占的比例最小。CYP7A1的基因多态性与儿童的血脂水平存在密切的关系。
Objective： To study the relationships between the polymorphism of CYP7A1 gene and the serum lipid levels in children in Hubei province. Methods： The study subjects consisted of 216 healthy children who were randomly selected from the school. The frequency distribution of the polymorphism in nucleotide A-204C of 7α-hydroxylase gene was detected using PCR-RELP. The serum lipid levels were determined using automatic bio- chemical analyzer. Results： The frequencies of AA, AC and CC genotypes were 6.0%, 26.4% and 67.6% respectively. There were significant differences in low density lipoprotein cholesterol （LDL-C）, total cholesterol （TC）, lipoprotein （a） levels among CC, CA and AA genotypes; in children with AA genotype, which were all lower than those in other genotypes （ P 〈 0.05 ） ; the levels of high density lipoprotein cholesterol （HDL-C） in children with CC genotype were higher than those in other genotypes （ P 〈 0.05）. There were no significant differences of the genotypes in triglycerides （TG） while significant differences among the three genotypes in ApoA. Conclusion： CC genotype is most common in children, AA genotype is least. There are close relationships between the polymorphism of the CYP7A1 gene and lipid levels in children.
出处
《海南医学院学报》
CAS
2008年第1期6-8,11,共4页
Journal of Hainan Medical University
关键词
胆固醇7α-羟化酶基因
单核苷酸多态性
血脂
儿童
Cholesterol 7α-hydroxylase （CYP7A1） gene
Single-stranded conformation polymorphism （SSCP）
Serum lipid
Children
