摘要
Lactoferricin是一种天然抗菌肽。试验进行了Lactoferricin基因的PCR合成,将其引入质粒,转化E.coliJM-109,并进行PCR鉴定、酶切鉴定及测序,成功构建了Lactoferricin基因克隆载体T-Lfn。
Lactoferricin is a kind of natural antibacterial peptide. In this study, the Lactoferricin gene was synthesized with PCR, linked with the plasmid of pMD18-T Vector, and transformed into E.coli JM-109. The identifications of PCR, enzymical degestion and sequencing were carried out, and the cloning vector of T-Lfn was successfully constructed.
出处
《东北农业大学学报》
CAS
CSCD
2008年第1期107-111,共5页
Journal of Northeast Agricultural University
基金
黑龙江省青年基金(QC06C015)
关键词
抗菌肽
乳铁素
载体构建
antimicrobial peptide
Lactoferricin
vector construction
