摘要
目的:建立以反相高效液相色谱法测定人血浆中盐酸表柔比星浓度的方法。方法:色谱柱为YWGC18,流动相为水-乙腈(12∶7,用85%磷酸调节体系pH为3.90±0.05),流速为1.0mL.min-1,检测波长为254nm。结果:人血浆中表柔比星检测浓度在0.12～4.80mg.L-1范围内线性关系良好(r=0.9984);最低血浆检测浓度为0.01mg.L-1;日内RSD<4.40%、日间RSD<5.60%,平均提取回收率为99.40%(RSD=4.02%);样品溶液在室温下12h内稳定。结论:本方法简便、灵敏、准确和重现性好。
OBJECTIVE：To determine the content of epirubicin in human plasma by RP-HPLC. METHODS：The chromatographic separation was performed on YWG C18 column .The mobile phase consisted of water- acetonitrile（ 12 ： 7,with pH adjusted to 3.90±0.05 using 85% phosophoric acid） at a flow rate of 1.0 mL· min^-1.The detective wavelength was set at 254 nm.RESULTS：The linear range of epirubicin was 0.12-4.80 mg · L^-1 （r = 0.998 4） with the lowest detective range of 0.01 mg ·L^-1.The intraday RSD was less than 4.40% and the inter- day RSD was less than 5.60% ,and the average recovery rate was 99.40% （RSD = 4.02%） .The sample solution was stable within 12 h under room temperature.CONCLUSION： The method is easy,precise and reproducible to operate and the result of the determination is accurate.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第5期346-347,共2页
China Pharmacy
