摘要
目的探讨盐酸戊乙奎醚（长托宁）对大鼠肝脏缺血．再灌注损伤的防治作用和可能机制。方法将24只大鼠随机均分为：A组，假手术对照组（n=8）；B组，单纯缺血．再灌注组（n=8）；C组，长托宁预处理组（n=8）。分别于肝脏缺血．再灌注模型成功后12h处死动物，留标本，以半定量逆转录．多聚酶链反应技术（RT-PCR）分析不同组肿瘤坏死因子-α（TIF-α）mRNA的表达，检测肝脏组织丙二醛（MDA）含量、血浆丙氨酸转移酶（AIJT）、肝组织病理学变化。结果肝脏缺血．再灌注后孙TNF-αmRNA的表达、血浆ALT和肝组织中MDA含量均显著升高。C组TNF-αmRNA的表达明显降低，ALT及肝组织中MDA也有不同程度的降低，分别为（91．30±9．75）U／L、（8．3±0．6）nmol／g，肝组织病理学损害明显减轻。结论长托宁可能通过抑制炎性细胞因子的释放及氧自由基的生成减轻大鼠肝脏缺血．再灌注损伤。
Objective To investigate effects of penehyclidine hydrochloride on hepatic ischemia/reperfusion injury and its mechanisms. Method Twenty-four male SD rots were randomly divided into 3 groups： group A, sham operation （n = 8）; group B, ischemia/reperfusion （IR） （n = 8） ; group C, pretreated with penehyclidine hydrochloride （n =8）. All rats were killed at 12 hours after operation, and the tumor necrosis factor-α （TNF-α） mRNA expression in liver was measured by RT-PCR. At the same time, the serum transaminases （ALT）, malonaidehyde （MDA） and the liver histopathology were detected. The data were expxessed as （x ± s ）, and were analyzed by using chi-square text and studentis t test with spss 11.0. A P valuue less than 0.05 indicated statistical significance. Results At 12 hours after repeffusion, the TNF-α mRNA expression, ALT and MDA were markedly increased. Compared with group A and group B, the expression of TNF-α mRNA, ALT （91.30± 9.75） U/L and MDA （8.3 ± 0.6） nmol/g were significantly lower in the group C （ P 〈 0.01 ）. The liver histopathology was also alleviated. Conclusions Penehyclidine hydrochloride could protect the liver from acute ischemia/reperfusion injury, and the mechanism might be related to its down-regulation of TNF-α and MDA expressions.
出处
《中华急诊医学杂志》
CAS
CSCD
2008年第1期50-53,共4页
Chinese Journal of Emergency Medicine
关键词
长托宁
肝脏
缺血-再灌注损伤
Penehyclidine Hydrochloride
Liver
Ischemia/Reperfusion injury
