摘要
New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.
New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer（FRET）. Nanoparticle A was doped with a dye complex which consisted of two dye molecules, fluorescein and tetramethylrhodamine. These dye molecules were first linked with avidin and biotin, respectively. They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET. Nanoparticle B was doped with fluorescein only. As a resuh of this combination, the nanoparticles emit double wavelengths when a single ex- citation wavelength is used. The resuhs show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2008年第1期83-85,共3页
Chemical Journal of Chinese Universities
基金
中国博士后科学基金(批准号:20040350561)资助
关键词
纳米粒子
双重对象
同时检测
荧光共振能量转移
Nanoparticle
Double targets
Simultaneous determination
Fluorescenee resonance energy transfer
