摘要
目的：建立高效液相色谱法（HPLC）测定人血清和置换液中关罗培南浓度的方法。方法：采用HPLC法，以依利特Hypersil ODS2柱（4.6mm×150mm，5μm）为色谱柱；甲醇-0.01mol·L^-1磷酸二氢钾缓冲液（9：91）为流动相，pH4.91；流速为1.4mL·min^-1检测波长为297nm。结果：血清中关罗培南的标准曲线方程为Y=17.027X-4.7541，r=0.9997，在0.5～80mg·L^-1范围内线性关系良好，平均回收率为100.2％，日内，日间RSD均小于7.2％；置换液中关罗培南的标准曲线方程为Y=18.931 X＋0.8928，r=0.9998.在0.5～80mg·L^-1范围内线性关系良好，平均回收率99.2％，日内、日间RSD均小于7.6％。结论：该方法灵敏准确，适用于临床药动学的研究。
OBJECTIVE To determine the concentration of meropenem in human serum and displacement liquid. METHODS HPLC analysis was carried out by using yilite hypersil ODS2 column（4. 6 mm×150 mm,5μm）and methanol-0.01 mol·L^-1 KH2 PO4 （9：91） as the mobile phase, pH4. 91;The flow rate was 1.4 mL·min^-1; The detecting wavelength was at 297 nm. RESULTS The linear range was within 0.5-80 mg·L^-1 of drug concentration in serum. The average recovery of meropenem was 100.2% ,RSD, of intra and inter-day were less than 7.2% ;The linear range was within 0.5-80 mg·L^-1 of drug concentration in displacement liquid. The average recovery of meropenem was 99. 2% ,RSD of intra and inter-day were less than 7. 6%, CONCLUSION This method is rapid and accurate,it can be used for the pharmacokinetic study of meropenem.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第12期1692-1694,共3页
Chinese Journal of Hospital Pharmacy
