摘要
目的调查临床分离的葡萄球菌对克林霉素诱导耐药状况和基因型特征。方法收集我院2004年分离到的70株红霉素耐药、克林霉素敏感和中介葡萄球菌临床分离株,双纸片法做克林霉素诱导耐药(D试验),PCR检测细菌的ermA、ermB、ermC和msrA基因。结果70株葡萄球菌中克林霉素诱导耐药32株(45.7%)。基因型分别是ermA1株,ermC33株,msrA18株,ermC+msrA5株,全部阴性的13株。结论我院葡萄球菌克林霉素诱导耐药率较高,且存在多种基因型,应提高对诱导耐药葡萄球菌的监测和控制。
Objective To investigate the prevalence and genotype of inducible clindamycin resistance in clinical Staphylococcus isolates. Methods Seventy isolates of erythromycin-resistant and clindamycin susceptible Staphylococcus isolates were collected from Shanghai Sixth People＇s Hospital in 2004, Double disc test （D-test） was used to detect the inducible clindamycin resistance, The errnA, errnB, ermC and msrA genes were amplified by PCR. Results Inducible clindamycin resistance was identified in 32 of 70 （45.7%） isolates. The identified genotypes were ermA （1）, errnC （33）, msrA （18）, errnC＋ msrA （5）. Thirteen isolates were negative for all these genes. Conclusions The rate of inducible clindamycin-resistant was high in Staphylococcus spp.. The Staphylococcus spp. of inducible clindamycin-resistant should be carefully monitored and effectively controlled.
出处
《中国感染与化疗杂志》
CAS
2008年第1期50-52,共3页
Chinese Journal of Infection and Chemotherapy
