摘要
目的建立Sprague Dawley(SD)大鼠体外循环模型,为体外循环脑、肺、肝、肾等重要脏器损伤的基础研究提供经济实用的动物模型。方法采用右颈内动脉插管灌注、右颈外静脉插管至右房引流方法进行SD大鼠闭胸体外循环转流,低温诱发室颤心脏停跳。将24只大鼠随机平均分为体外循环120min组、深低温低流量60min组、深低温停循环60min组,术中进行有创血压、心电图监测和动脉、混合静脉血气分析。结果24只大鼠均顺利建立体外循环模型,血气指标均正常范围内,摆脱体外循环时平均血压维持50mmHg以上,停体外循环后心肺功能均顺利恢复。结论本实验建立大鼠闭胸不同体外循环模型是切实可行的,该模型是研究体外循环生理的一个可信赖的平台,有利于研究体外循环后多脏器损伤的病理生理变化。
Objective To establish an animal model of closed chest cardiopulmonary bypass （CPB） in Sprague-Dawley （SD） rats. Methods The SD rats were assigned to undergo CPB via right carotid and jugular cannulation,and self-designed CPB circuit. The cardiac arrest was induced by hypothermal ventricular fibrillation. Twenty-four rats were equally divided into three groups which underwent CPB for 120 minutes,deep hypothermic low flow （DHLF） bypass 60 minutes,deep hypothermic circulatory arrest （DHCA） 60 minutes individually. During bypass, invasive arterial blood pressure, electrocardiograph and arterial and mixed venous blood gas analysis were monitored. Results The CPB was performed successfully in all the rats. The blood gas analysis results were all maintained in normal range and the mean arterial pressure was over 50 mmHg when off-pump. The cardiac and respiratory functions recovered after the rat weaned from the CPB. Conclusions The animal model of closed chest CPB can be established successfully in rats with excellent survival. This model is a stable platform for the study of the pathophysiology of CPB and post-CPB multiple organ dysfunctions.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2008年第1期111-115,共5页
Fudan University Journal of Medical Sciences
关键词
动物模型
体外循环
大鼠
models
animal
cardiopulmonary bypass
rats
