摘要
目的:探讨骨涎蛋白(bone sialoprotein,BSP)单克隆抗体对亲骨转移乳腺癌细胞(MDA-MB-231-BO)与骨基质黏附的抑制作用。方法:免疫组化方法检测MDA-MB-231-BO、肺癌细胞SPC-1、大肠癌细胞LOVO、乳腺癌细胞BSP的表达;体外骨基质黏附实验检测肿瘤细胞与骨基质的黏附,以及BSP单克隆抗体对MDA-MB-231-BO细胞与骨基质黏附的抑制;以ELISA法检测肿瘤细胞中生长因子TGF-β1分泌的变化。结果:免疫组化结果表明,MDA-MB-231-F10细胞表达BSP,SPCA-1和LOVO细胞不表达BSP;MDA-MB-231-BO细胞黏附骨基质数明显大于SPCA-1细胞和LOVO细胞(P<0.01);BSP抗体对MDA-MB-231-BO细胞和骨基质黏附具有特异抑制作用,随着抗体质量浓度升高(25、50、100、200μg/ml),其抑制率分别为22.1%、35.0%、39.2%、41.9%,抑制率与抗体浓度呈正相关;同时BSP抗体能特异性抑制MDA-MB-231-BO细胞与骨基质黏附所介导TGF-β1的释放。结论:BSP单克隆抗体对MDA-MB-231-BO细胞和骨基质黏附具有特异抑制作用,提示BSP抗体可抑制乳腺癌特异性骨转移。
Objective： To determine the inhibitory effects of anti-bone sialoprotein （BSP） antibody on human breast cancer cells adhering to bone matrix in vitro. Methods： Expression of BSP in 3 different cancer cell lines （bone seeking breast cancer cell MDA-MB-231-BO, lung adenocarcinoma cell SPCA-1 and colon carcinoma cell LOVO）was detected immunohistochemically. Cells adhering test was carried out to investigate the adhering of the 3 different cancer cell lines to mouse bone matrix in vitro and the inhibitory effect of anti-BSP antibody on MDA-MB-231-BO cells adhering to mouse bone matrix; Enzyme-linked immunosorbent assay was carried out for quantitative detection of TGF-β. Results： BSP immunostaining was positive in MDA-MB-231-BO cells and negative in SPCA-1 and Lovo cells. The number of MDA-MB- 231-F10 cells adhering to mouse bone matrix was significantly more than SPCA-1 or Lovo cells（ P 〈 0.01 ）. Anti-BSP antibody specifically inhibited MDA-MB-231-BO cells adhering to bone matrix, and the inhibitory rate of anti-BSP antibody was in a concentration-dependent manner. Anti-BSP antibody also inhibited TGF-β1 secretion after MDA-MB-231-cells adhering to bone matrix. Conclusion： Anti-BSP antibody can specifically inhibit breast cancer cells adhering to mouse bone matrix, which suggests that anti-BSP antibody may inhibit the metastasis of breast cancer to bone.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2007年第6期536-539,共4页
Chinese Journal of Cancer Biotherapy
基金
广东省自然科学基金重点项目资助(NO.06104396)~~
