摘要
目的探讨TPO模拟肽(thrombopoietin mimetic peptide,TMP)与人生长激素(growth hormone,GH)的重组融合蛋白rTMP-GH对体外培养巨核系细胞增殖的影响,以及对血小板生成有显著调控作用的球蛋白转录调节因子(globin transcription factor-1,GATA-1)表达的调控作用。方法体外培养Dami细胞,100ng/ml的重组融合蛋白rTMP-GH作用7d后,观察巨核细胞集落形成能力,分析rTMP-GH对Dami细胞增殖的影响。同时,采用Western blot和RT-PCR方法分别检测Dami细胞中GATA-1的蛋白水平和mRNA表达变化,探讨rTMP-GH对巨核细胞生成血小板的调节作用。结果rTMP-GH处理后Dami细胞的集落形成能力显著增强,转录因子GATA-1的mRNA和蛋白表达水平显著上调,其调节作用明显强于空白对照组和GH组。结论rTMP-GH能够刺激巨核细胞增殖分化和上调GATA-1的表达,具有促进血小板生成的作用。
Objective To investigate the effects of rTMP-GH, a recombinant fusion protein of thrombopoietin mimetic peptide （TMP） and human growth hormone （GH）, on the proliferation and thrombocytopoiesis of cultured megakaryocytes. Methods After being treated with 100 ng/ml of rTMP-GH for 7 d, Dami cells, a kind of megakaryocyte cell line, were analyzed by observing the numbers of colony forming unit. Meanwhile, Western blotting and RT-PCR were applied to detect the expression changes of globin transcription factor-1 （ GATA-1 ）, which is a main regulator of thrombocytopoiesis in megakaryocytes. Results The numbers of col- ony forming unit were markedly increased in cuhured Dami cells incubated with rTMP-GH. Compared with normal control and GH treatment groups, both the mRNA and protein levels of GATA-1 were up-regulated significantly in Dami cells treated with rTMP-GH. Conclusion rTMP-GH has a strong ability to promote the prolifer- ation and thrombocytopoiesis of megakaryocytes.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第1期1-4,共4页
Journal of Third Military Medical University
基金
国家高技术研究发展计划(“863”计划)(2007AA02Z152)
全军医学科研“十一五”科技攻关项目(06G076)
全国博士学位论文作者专项资金(200362)~~
关键词
rTMP-GH
Dami细胞
增殖
GATA-1
thrombopoietin mimetic peptide-growth hormone
Dami cells
Proliferation
GATA-1
