摘要
目的检测非小细胞肺癌(NSCLC)组织中HER2和p300/CBP蛋白的表达,并探讨其临床意义。方法应用免疫组织化学Evision二步法,检测71例NSCLC患者肿瘤组织和20例正常肺组织标本中HER2和p300/CBP蛋白表达水平。结果正常肺组织未见HER2和p300/CBP蛋白过表达,NSCLC中存在HER2和p300/CBP蛋白过表达,过表达率分别为34%和76%(24/71,54/71)。肿瘤组织中HER2蛋白表达情况与肿瘤组织学类型、TNM分期、淋巴结转移情况有相关性(P=0.039、0.003、0.033),与肿瘤细胞分化程度、性别、年龄未见相关性。p300/CBP与肿瘤各临床病理参数无相关性。结论NSCLC患者肿瘤组织中HER2、p300/CBP蛋白过表达。HER2过表达程度与NSCLC淋巴结转移、TNM分期和组织学类型有相关性。p300/CBP与肿瘤各临床病理参数无相关性。
[ Objective] To detect the expression of HER2 and p300/ CBP protein in no-small cell lung cancer （NSCLC） and analyze the relationship between their expression and clinic significance. [ Methods] The protein expressions of HER2 and p300/CBP in 71 NSCLC tissues and 20 normal lung tissues were by immunochemistry. [ Results] The overexpression of HER2 and p300/CBP in normal lung tissue were not found, the protein levels of HER2 and p300/CBP in NSCLC were generally detected with which high expression of HER2 was detected as 34% （24/71）and 76% for p300/ CBP（54/71 ）. The rate of protein expression in lung adenocarcinoma was 45.5% （20/44） while expression rate was 19.9% （4/21） in the squamous carcinoma. These differences have statistics meanings （P 〈 0. 05 ）. The overexpression rate of lymph transfer group （43.2%, 19/44） was remarkably higher than the group, which has not been transferred as well （18.5%, 5/27）. The two groups had conspicuous significance （P 〈 0. 05）. HER2 overexpression correlated with TNM staging, the overexpression of HER2 in TNM Ⅲ ＋ Ⅳ group was significantly higher than that in TNM Ⅰ ＋ Ⅱ group （P 〈0.05） . [ Conclusions] HER2 overexpression correlates with pathological type, TNM staging and lymph node metastasis , and is a poor prognostic factor for NSCLC . p300/ CBP overexpression was not correlated with all the pathological parameters statistically. There was no correlation between HER2 and p300/ CBP expression.
出处
《山东医药》
CAS
北大核心
2007年第36期27-29,共3页
Shandong Medical Journal
基金
深圳市科技计划项目(200404027)
