摘要
采用PCR-DGGE法对从福建稻田取得的8个固氮细菌分离物进行分子鉴定,通过测序比对,发现它们与中慢生根瘤菌(Mesorhizobiumsp.)、鞘氨醇单胞菌(Sphingomonassp.)、根瘤菌(Rhizobiumsp.)、慢生根瘤菌(Bradyrhizobiumsp.)、克雷伯氏杆菌(Klebsiellasp.)、伯克霍德尔氏菌(Burkholderia cepacia)、假单胞菌(Pseud-omonassp.)、诺卡氏菌(Nocardioides)和红球菌(Rhodococcussp.)9个属的固氮细菌具有高度同源性。
Eight bacterial isolates, which originated from paddy fields in Fujian, were analyzed using 16SrDNA- PCR-DGGE. Through sequencing and Blast analysis, 9 N2-fixing bacteria strains were believed to belong to Mesorhizobium sp. , Sphingomonas sp. , Rhizobium sp. , Bradyrhizobium sp. , Klebsiella sp. , Burkholderia cepacia, Pseudomonas sp. , Nocardioides and Rhodococcus sp. , due to their high homology with the related accessions in Genbank.
出处
《福建农业学报》
CAS
2007年第4期346-349,共4页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划项目(2001Z026)
