摘要
目的:筛选土茯苓总黄酮提取的优化工艺。方法:应用微波辅助提取技术,通过单因素和正交实验考察提取时间、微波功率、乙醇浓度、固液比和提取温度对土茯苓总黄酮提取率的影响。结果:提取土茯苓总黄酮的优化工艺条件为:提取时间5 min,微波功率600W,乙醇浓度60%,固液比1∶30,提取温度80℃。结论:优化后的工艺稳定,微波辅助提取土茯苓总黄酮提取率高、时间短。按照优化后工艺条件实验,土茯苓总黄酮提取率、总黄酮得率和粗黄酮粉中总黄酮含量分别为93.2%、2.66%和32.6%。
Objective： To optimize the process of extracting flavonoids from Smilax glabra. Methods： Flavonoids were extracted from Smilax glabra by microwave-assisted method, and the extracting time, microwave power, ethanol concentration, solid-solvent ratio and extracting temperature were optimized through single factor experiment and orthogonal test. Results： The optimun process parame- ters of extracting Navonoids were as follows： the extracting time, microwave power, ethanol concentration, solid-liquid ratio and extracting temperature were 5 minutes, 600W, 60% , 1 ： 30 and 80℃, respectively. Conclusion ： The proposed method has been applied stably and reliably to the extraction of flavonoids from Smilax glabra which has the advantages of high recovery and short extraction time. The recovery, the yield and the content of falvonoids are 93.2% , 2. 66% and 32. 6% , respectively.
出处
《中药材》
CAS
CSCD
北大核心
2007年第12期1591-1595,共5页
Journal of Chinese Medicinal Materials
基金
湖北省自然科学基金创新群体项目(2006ABC014)
武汉工程大学校长基金(2006038)
关键词
微波辅助提取
土茯苓
总黄酮
Microwave-assisted extraction
Smilax glabra Roxb
Flavonoids
