摘要
目的:检测乳腺癌患者外周血、肿瘤组织中hSTC-1mRNA的表达,判断外周血微转移状况,探讨微转移与肿瘤恶性程度之间的关系。方法:采用RT-PCR法检测乳腺癌组织、外周血中hSTC-1的mRNA表达,用免疫组化法检测乳腺肿瘤组织中ER、PR及VEGF的表达。结果:hSTC-1mRNA在乳腺癌患者癌组织中的阳性率为93.3%(28/30),在外周血中的阳性率为37.3%(19/51);17例非肿瘤成年女性患者外周血中未检测到hSTC-1的mRNA。血液中的hSTC-1的mRNA表达与多个临床病理因子有相关性(P<0.05),包括:肿瘤大小、腋淋巴结转移、TNM分期、ER、VEGF等;结论:乳腺癌患者外周血检测到hSTC-1基因的表达,说明乳腺癌已发生血行微转移,乳腺癌患者外周血hSTC-1的mRNA表达与多个临床病理因子有关;这些因子决定乳腺癌的预后,因此hSTC-1可以作为检测乳腺癌外周血微转移的一种新的分子标记。
Objective ：To detect the expression of human stanniocalcin-1 （ hSTC-1 ） mRNA in peripheral blood and tumor tissues of breast cancer patients, determine micrometastases in peripheral blood, and study the relationship between the micrometastases and malignant degree of breast cancer. Methods： The expression of hSTC-1 mRNA in peripheral blood and breast cancer tissues was detected by RT-PCR. The expression of estrogen receptor （ER）, progesterone receptor （PR）, and vascular endothelial growth factor （VEGF） in breast cancer tissues were detected by immunohistochemical method. Results： Expression of hSTC-1 mRNA was identified in breast cancer tissues from 28 of 30 patients （93.3%） and in peripheral blood from 19 of Sl patients （37.3%）. Expression of hSTC-1 mRNA was not detected in the peripheral blood from 17 women without breast cancer. Expression of hSTC-1 mRNA in the peripheral blood significantly correlated with multiple clinicopathological factors （ P 〈 0.05 ）, including tumor size, metastasis of lymph nodes, TNM stage, and the expression of ER and VEGF. Conclusion： The expression of hSTC-1 mRNA in the peripheral blood of breast cancer patients suggests the occurrence of micrometastasis. It significantly correlates with multiple clinicopathological factors which determined the prog- nosis of breast cancer. So hSTC-1 is proposed as a new marker for detecting micrometastasis of breast cancer in the peripheral blood.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第12期977-980,共4页
Tumor
基金
南通市科技局资助项目(编号:S5052)
关键词
乳腺肿瘤
肿瘤转移
反转录聚合酶链反应
人类斯钙素-1
Breast neoplasms
Metastasis
Reverse transcriptase polymerase chain reaction
Human stanniocalcin-1
