摘要
目的:建立苦苣菜总黄酮的含量测定方法,为考察其内在质量提供依据。方法:通过不同提取方法,采用紫外-可见分光光度法测定苦苣菜总黄酮的含量,检测波长为510nm。结果:采用70%乙醇超声提取苦苣菜总黄酮,NaNO2-Al(NO3)3-NaOH为显色系统的含量测定方法,线性范围13.2～79.2μg/mL,r=0.9998,平均加样回收率为98.27%,RSD=1.67%(n=6),测得样品中总黄酮的含量为2.12%。结论:含量测定方法操作简便,快捷,重现性好,与传统提取法相比,超声提取法具有快速、高效等显著优点。
Objective： To establish a method of determination of total flavonoids in Sonchus oleraceus and to provide basis for the study of its internal quality. Methods ： The content of total flavonoids in Sonchus oleraceus was determined by UV- Vis spectroscopy with a detection wavelength at 510nm by different extraction methods. Results： The total flavonoids, which was extracted from Sonchus oleraceus by 70% alcohol, was determined by ultraviolet spectroscopy with NaNO2 - Al （ NO3 ） 3 - NaOH system, the liner range varied from 13.2 - 79.2μg/mL （ r = 0.9998 ）, average recovery was 98.27%, RSD = 1.67% （ n = 6 ）. The average content of total flavonoids in Sonchus oleraceus was 2. 12%. Conclusion： The method was easily operated and repreducible. Compared with traditional extraction, the ultrasonic extraction had higher extraction efficiency.
出处
《中华中医药学刊》
CAS
2008年第1期218-220,共3页
Chinese Archives of Traditional Chinese Medicine
关键词
苦苣菜
总黄酮
分光光度法
Sonchus oleraceus
total flavonoids
spectroscopy
