摘要
目的建立适合移植实验使用的裸鼠造血微环境损伤动物模型。方法分别用3.5Gy、5Gy、6.5Gy、8Gy4个60Coγ辐射剂量照射裸鼠,检测经过辐照后各组裸鼠1、3、5、7、14、21 d血象变化,检测辐射后1、7、14、21 d裸鼠骨髓基质CFU-F形成情况,用流式细胞仪检测辐射后1、7、14、21 d骨髓基质细胞VCAM-1的表达。结果8.0 Gy辐射剂量裸鼠死亡率100%;3.5 Gy、5 Gy、6.5 Gy辐射剂量组裸鼠的造血损伤均能在辐射后21天之内自我恢复;6.5 Gy组骨髓CFU-F于移植后7 d(22±6)、14d(33±6)较3.5 Gy、5 Gy组均具有显著降低(P<0.01),VCAM-1表达于7 d(21.67±7.45)、14d(22.33±9.07)、21d(23.33±4.51)较对照组显著降低(P<0.01)、较3.5 Gy组差异有显著性(P<0.05),21d(23.33±4.51)较5 Gy组(34.00±4.36)差异有显著性(P<0.05)。结论6.5Gy是裸鼠造血微环境损伤实验动物模型的适宜辐射剂量。
Objective To establish the hematopoietic inductive microenvironment （HIM） damage model for the transplant experiment in nude mice. Methods Irradiate the experimental nude mice by 3.5GY, 5GY, 6- 5GY and 8GY ^60Coγ respectively and detect the changes of hemogram at 1, 3, 5, 7, 14, and 21 days and the formation of CFU-F in marrow stroma at 1,7,14, and 21 days, and the expression of VCAM-1 at 1, 7, 14, and 21 days by flow cytometry. Results Irradiated with 8Gy, the death rate of nude mice was 100%. All the nude mice in 3.5Gy group, 5Gy group and 6.5Gy group could be recovered by themselves within 21 days after radiation. Moreover in the 6.5Gy group, the CFU- F was markedly lower at 7d（22±6） and 14d（33±6） than 3.5Gy and 5Gy groups（P〈0.01）. The expression of VCAM- 1 at 7d（21.67±7.45）, 14d（22.33±9.07）,and 21d（23.33±4. 51） was significantly lower than the control group （P〈 0.01）, and different from the 3.5Gy group（P〈0. 05）. The expression of VCAM-1 was significantly different between 5Gy group （34.00±4.36） and at 21d （23.33±4. 51 ,P〈0.05）. Conclusion 6. 5Gy is the suitable radiation dose for the HIM damage model in nude mice.
出处
《西部医学》
2008年第1期38-41,共4页
Medical Journal of West China
基金
国家自然科学基金资助课题(No.30070327
30500220)
第三军医大学科研基金资助项目(No.XG200347)
关键词
造血微环境
裸鼠
动物模型
Hematopoietic inductive microenvironment
Nude mice
Animal model
