摘要
目的探讨P27蛋白表达与宫颈鳞状细胞癌发生、发展的关系。方法采用SP法免疫组化分别检测宫颈鳞状细胞癌、癌旁高级别鳞状上皮病变、癌旁正常鳞状上皮及非癌旁正常鳞状上皮组织中P27蛋白的表达。结果宫颈鳞状细胞癌、癌旁高级别鳞状上皮病变、癌旁正常鳞状上皮及非癌旁正常鳞状上皮组织中P27阳性表达率分别为35.00% (21/60)、38.89%(14/36)、77.78%(42/64)、80.00%(8/10),其中宫颈鳞状细胞癌及癌旁高级别鳞状上皮病变P27阳性表达率与癌旁及非癌旁正常宫颈鳞状上皮相比差异均有显著性(P<0.05或0.01)。P27阳性表达率下降与组织学分级、肿瘤大小、肿瘤浸润及琳巴转移有关(P均<0.01),与年龄无关(P>0.05)。结论P27在宫颈鳞状细胞癌的发生、发展过程中起重要的作用,体现在P27蛋白表达降低或缺失时易导致宫颈鳞状细胞癌的发生和转移,因此检测P27蛋白表达可作为宫颈鳞状细胞癌的发生、发展及预后评估的一个有用指标。
Objective To investigate the relationship between the expression of P27 protein and the oncogenesis and development of cervical squamous carcinoma （CSC）. Methods The expression of P27 was detected by using SP immunohistochemical staining in paraffin sections from cases of CSC, high - grade squamous intraepithelial lesion （HSIL） and corresponding normal squamous tissues （CNST） closely adjacent to carcinoma, and other normal squamous tissues （ONST）. Results The positive rates of P27 protein expression in SCS, HSIL, CNS＇T and ONST were35% （21/60）, 38.89% （14/36）, 77.78% （42/54） and 80% （8/10）, respectively. The comparison of positive rates among SCS, HSIL and CNST, ONST showed significant differences （ P 〈 0.05 or 0.01）. The decreased expression of P27 protein was associated with histologic grading, tumor size, invasion and metastasis of SCS （ all P 〈 0.01）, and it was not associated with age （ P 〉0.05）. Conclusion P27 protein might play an important suppressing role in the genesis and development of CSC, the downregulated expression of CSC might easily lead to lymphnode metastasis and tumorous invasion, expression of P27 could be helpful in predictin the biological behavior and prognosis of CSC.
出处
《右江民族医学院学报》
2008年第1期27-29,共3页
Journal of Youjiang Medical University for Nationalities
关键词
宫颈肿瘤
癌
鳞状细胞
P27蛋白
cervical neoplasms
carcinoma, squamous cell
P27 protein
