摘要
用保加利亚乳酸杆菌发酵和用胰蛋白酶、中性蛋白酶、木瓜蛋白酶酶解超微粉碎牦牛骨泥,测定骨泥中的游离钙(Ca2+)、氨基酸态氮含量和pH值的变化。结果表明,经保加利亚乳酸杆菌发酵36h后,骨泥中游离钙的含量从12.3mg/100g增加到2145.3mg/100g,而氨基酸态氮的含量从117.7mg/100g增加到233.4mg/100g;经胰蛋白酶、中性蛋白酶和木瓜蛋白酶分别酶解4h后,游离钙的含量从23.3mg/100g分别增加到89.3mg/100g,62.3mg/100g和52.3mg/100g,氨基酸态氮则从106.5mg/100g分别增加到134.5mg/100g、149.4mg/100g和154.1mg/100g。通过比较显示,利用乳酸菌发酵处理骨泥后,游离钙含量是酶解处理骨泥的24倍,氨基酸态氮含量则为酶解处理的1.5倍。因此发酵比酶解处理能得到更多的游离钙和氨基酸态氮。
Smashed yak bone paste was subjected to the fermentation by Lactobacillus bulgarian or the enzymolysis by trypsinase, dispase or caroid, respectively, followed by the determination of dissociative calcium （Ca^2＋） content, amino acid nitrogen content and pH. The results showed that, after fermentation by L. Bulgarian for 36 h, the Ca^2＋ content in bone paste increased dramatically from 12,3 mg/100g to 2145.3 mg/100g, and the amino acid nitrogen content increased from 117.7 mg/100g to 233.4 mg/100g. After enzymolysis by trypsinase, dispase, or caroid for 4 h respectively, the Ca^2＋ content increased from 23.3 mg/100g to 89.3 mg/100g, 62.3 mg/100g and 52.3 mg/100g, respectively, while the amino acid nitrogen content increased from 106.5 mg/100g to 134.5 mg/100g, 149.4 mg/100g and 154.1 mg/100g, respectively. With the comparison of fermentation and enzymolysis treatment, it was indicated that the Ca^2＋ content and amino acid nitrogen content were raised 24 times higher and 1.5 times higher by fermentation than by enzymolysis, respectively. That was, more Ca^2＋ and amino acid nitrogen could be obtained by fermentation than by enzymolysis.
出处
《中国酿造》
CAS
北大核心
2008年第1期41-43,共3页
China Brewing
关键词
牦牛骨泥
发酵
酶解
游离钙
氨基酸态氮
yak bone paste
fermentation
enzymolysis
dissociative calcium
amino acid nitrogen
