摘要
目的:研究大豆磷脂对小鼠学习记忆和海马二十二碳六烯酸(C22:6,DHA)和二十碳五烯酸(C20:5,EPA)等脂肪酸含量的影响,探讨其改善学习记忆的相关机制。方法:48只小鼠随机均分成4组,分别用不同剂量的大豆磷脂,为低(2.5g/kgbw)、中(5.0g/kgbw)、高剂量(10.0g/kgbw)灌胃,对照组灌胃去离子水,连续灌胃30d,测定小鼠学习记忆能力后处死,取小鼠海马,经一系列处理后采用高效液相色谱测定海马中DHA和EPA等脂肪酸含量。结果:(1)与对照组比较,高、中剂量组小鼠学习记忆能力均显著增强;低剂量组小鼠记忆力明显改善;(2)中、高剂量组小鼠海马DHA含量较对照组显著提高,而EPA下降明显;(3)高剂量组小鼠海马油酸(C18:1)、二十二碳六烯酸(C22:6)显著上升,花生四烯酸(C20:4,AA)有上升趋势,但无显著差异。二十碳五烯酸(C20:5)、亚油酸(C18:2)含量则明显下降。结论:大豆磷脂明显提高小鼠学习记忆能力,可能与大豆磷脂改变小鼠海马脂肪酸的构成有关。
Objective： To study the effects of soybean phospholipids on learning and memory abilities and contents of fatty acids in hippocampus of mice and its mechanism. Method： Mice were randomly divided into four groups： low dose （2.5g/kg）, middle dose （5.0g/kg） and high dose （10.0g/kg） groups of soybean phospholipids and control group. The treatment groups were fed with soybean phospholipids every day while the control group with distilled water for 30 d. After learning and memory abilities were measured, the mice were killed and contents of fatty acids in hippocampus of mice were determined by high performance liquid chromatography （HPLC） . Results： （1） The learning and memory abilities of mice in middle and high dose groups were significantly improved, but only memory abilities in low dose groups were stronger than control group. （2） The contents of protein in hippocampus in middle and high dose groups were increased extremely. （3） The contents of oleic acid （C18：1） and docosahexaenoic acid （DHA,C22：6） were obviously enhanced in hippocampus of high dose group; but decreased in eicosapentaenoic acid （EPA,C20：5） and linoleic acid （C18：2）. The content of DHA was increased and EPA decreased significantly in middle dose group.The increase of arachidonic acid （C20：4） was not significant. Conclusion. Soybean phospholipids may improve learning and memory abilities, which might contribute to change of fatty acids contents in hippocampus of mice.
出处
《营养学报》
CAS
CSCD
北大核心
2007年第6期540-543,共4页
Acta Nutrimenta Sinica
基金
浙江省大型测试基金(No.04067)
