摘要
本实验利用80对SSR引物对20个玉米自交系进行扩增,综合考虑扩增带的清晰度及多态性、条带多少、引物重复性高低,筛选出Phi080,Phi123,Umc1061,Phi126,Phi065,Dupssr13,Phi102228,bnlg240,Phi083等9对引物,构建了20个玉米自交系的指纹图谱。其中有7个材料各用1对引物就可确定其特征性谱带,有3个材料各需用2对引物组合就能区分,有2个材料需4对引物组合方能予以有效区分,其余材料则需8对引物组合才可区分。本实验还对这9对引物的重复性作了验证,再次证明利用SSR标记构建玉米自交系指纹图谱是可行的,也是可靠的。
Fingerprints were established for 20 maize inbred lines by SSR markers. 20 maize inbred lines were used to screen 80 SSR primers and finally a set of primer pairs fitting for establishing maize inbred lines DNA fingerprinting were conformed ,which were Phi080, Phi123, Umel061, Phi126, Phi065, Dupssr13, Phi102228, bnlg240and Phi083. At the same time the uniformity of repetitive experiments indicated it was a reliable method to construct maize inbred lines fingerprinting by SSR.
出处
《西南农业学报》
CSCD
2007年第6期1172-1175,共4页
Southwest China Journal of Agricultural Sciences
基金
北京农业育种基础研究创新平台
北京市自然科学"中国玉米标准DNA指纹库构建及关键技术研究"课题(YZ-PT02-06)
"四川省应用基础研究计划课题(04JY029-004-1)"
