摘要
目的探讨长春新碱对人胃癌细胞杀伤作用和GSTπ表达的影响。方法利用人胃癌细胞株(型)进行培养,加入硫酸长春新碱注射液,5 h和10 h后进行光镜和电镜观察和SP免疫组织化学染色。结果10 h组外型畸变和细胞器破坏严重;5 h组GSTπ表达位置和灰度发生变化以核最明显。结论长春新碱对人胃癌细胞的杀伤作用和对GSTπ基因表达的影响与作用时间有关。
Objective To study the killing function to person＇s gastric cancer cells and the expressions of GST with vineristine. Methods As persons gastric cancer cells being educated, vincristine for junction were injected to educate continuously for 5 h or 10 h, then the person＇s gastric cancer cells were observed under optical microscope and transmission electron microscope, and stained with immunohistochemical method. Results The configuration was aberrant and the cell organs were seriously destroyed in the group for 10 hours, The changes of position and ash degree were exited in expression of GSTπ, and was the most obvious in nucleas. Conclusion The killing function to gastric cancer cells and the expression of GSTπ with vincristine has a significant relation to the acting time.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第12期3036-3037,共2页
Lishizhen Medicine and Materia Medica Research
关键词
长春新碱
胃癌
谷胱甘肽S转移酶
免疫组织化学染色
Vincristine （VCR） , Gastric cancer, Glutathion transfer enzyme（ GSTπ） , Immunohistochemical method
