摘要
参照GenBank中内源性绵羊肺腺瘤病毒enJS56A1株pol基因序列设计了2对引物。应用PCR技术特异性地扩增出病毒的pol基因片段,将其克隆到PMD19-T载体后测序。应用计算机软件将测定序列与内源性南非代表毒株enJS56A1(AF153615)的pol基因序列比较,核苷酸同源性为99.3%,推导出的氨基酸同源性为95.0%。与外源性南非代表株JSRV-SA(M80216)的pol基因序列比较,核苷酸同源性为99.0%,氨基酸同源性为99.3%。这也是我国首次报道的内源性绵羊肺腺瘤病毒pol基因序列,为我国科研工作者进行更深入的研究奠定基础。
According to the published pol gene sequence of enJS56A1 strain in GenBank, two pairs of primers were designed. A pol gene was amplified by PCR and then was cloned into PMD19-T vector and then sequenced. The acquired nucleotide sequences were analysed by computer softwares. As a result, The nucleotide and amino acid sequences of NM strain pol gene were compared with the counterpart sequences of South Africa enJS56A1 strain（AF153615） and South Africa JSRV-SA strain （M80216）. The nucleotide and amino acid homology of pol gene were 99.3%,95.0% and 98.8%,98.8%, respectively. This is the first nucleotide sequence of enJSRV reported in China. These results provide information for future developments on OPA.
出处
《中国兽医杂志》
CAS
北大核心
2007年第12期8-10,共3页
Chinese Journal of Veterinary Medicine
基金
国家自然科学基金(30560108)
