摘要
考察不了同温度(10℃～30℃)对硅藻(Nitzschia laevis)合成二十碳五烯酸(EPA)的影响,对不同温度下的发酵过程进行动力学特性分析。在此基础上,提出了EPA合成的变温培养方法:延滞期及对数期初期温度控制在25℃,从对数期中期开始在20℃条件下进行培养。采用此变温培养进行发酵,EPA的含量和产量分别达到了6.00%和291.60mg/L,较采用单一温度(25℃)发酵的最大值分别提高了24.07%和18.81%。
Fermentation for Eicosapentaenoic Acid （EPA） production by Nitzschia laevis at various temperature between 10℃ were also analyzed. Based and 30℃ was investigated and the dynamics characteristics during fermentation process on the results, a varying temperature nursing method of two stage control strategy is stage, which comprises the delay phase and the initial index phase, the temperature is maintained at 25℃ ; then the temperature is shifted to 20℃ and kept up till the end of the fermentation process. By this method,a EPA content of 6.0% and a yield of 291.60 mg/L have been gained. These are 24.07% and 18.81% higher than that of fixed temperature （25 ℃ ） fermentation, respectively.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第12期57-60,共4页
China Biotechnology
基金
天津市应用基础研究项目基金资助项目(05YFJZJC00700)
