摘要
供试的8种无机盐中,MgSO_4的单因子效应最好,可促进噬夏孢欧文氏菌(Erwinia uredovora)的生长和类胡萝卜素合成。在供试的4种碳源中,葡萄糖是最适碳源。噬夏孢欧文氏菌不能利用无机氮源,在供试的有机氮源中以酵母膏最有利于类胡萝卜素合成。培养基中的含碳量保持在2．45～4．90g/L,含氮量保持在1．60～2．40g/L时,对噬夏孢欧文氏菌生长和类胡萝卜素合成均为有利,培养基的最适C:N为2．5:1。类胡萝卜素稳定性的初步实验证明,所得到的类胡萝卜素丙酮溶液在常温下稳定性较差,容易分解。高效液相色谱法(HPLC)测定结果表明,从噬夏孢欧文氏菌细胞得到的天然色素成分比较复杂,在菌体中共检测到11种色素成分。
For the synthesis of carotenoids bacterium Erwinia uredovora. MgSO4 had the best positive effects among 8 inorganic salts, which can promote the growth of Erwinia uredovora and increase its pigments yield. All of the four carbon sources （glucose, sucrose, maltose and glycerol） could promote the cell biomass and biosynthesize the carotenoids with glucose as the best. As for the 5 nitrogen sources, yeast extract served as the best nitrogen source for the growth and pigments accumulation, followed by peptone and beef extract. Erwinia uredovora could not utilize urea and ammonium nitrate. The carbon and nitrogen cencentrations the culture media are 2.45-4.90g/L and 1.60-2.40g/L respectively, beneficial to both the cell growth and carotenoids production. The optimal C：N ratio is 2.5：1. It showed that the stability of carotenoids the acetone extract of Erwinia uredovora becomes worse and degrades fast during the first 24h under the room temperature. 11 pigments are detected from Erwinia uredovora.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第4期183-186,共4页
Food Science
基金
山东省博士基金(2003)
青岛市自然科学基金项目(05-1-JC-91)
